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US 12,523,664 B2Granted
Sensor chip and methods thereof
AssigneeNational University of Singapore
InventorsHuilin Shao, Zhi Jun Carine Lim, Yan Zhang
Life of this patent
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Abstract
The present disclosure relates generally to a sensor chip and methods for the detection of an analyte. In particular, the disclosure relates to a sensor chip for detecting an analyte in a subject suffering from a neurodegenerative disease. The sensor chip comprises a conductive layer on a membrane support layer, wherein a plurality of apertures extend through the conductive layer and the membrane support layer and are arranged such that illumination of the conductive layer and/or the membrane support layer produces a surface plasmon resonance.
Claims21
An imaging system comprising:
- a) a light source;
- b) a detector;
- c) a sensor chip comprising:
- i) a conductive layer on a membrane support layer wherein the conductive layer and the membrane support layer are disposed on a substrate, the substrate having a void formed therein in a region adjacent to a plurality of apertures to enable illumination of the membrane support layer to produce a surface plasmon resonance;
- ii) the plurality of apertures extending through the conductive layer and the membrane support layer, wherein the plurality of apertures are arranged such that illumination of the membrane support layer produces the surface plasmon resonance;
- and
- iii) a first recognition molecule that bids specifically to a first analyte, the first recognition molecule being immobilized onto a surface of the conductive layer;
- and
- d) a second recognition molecule that binds specifically to the first analyte or to a second analyte that is co-localized on a cell or exosome with the first analyte, wherein the second recognition molecule is coupled to a signal amplification moiety, and wherein the signal amplification moiety is capable of inducing the formation of an insoluble aggregate of increased optical density relative to that of the first analyte when the first analyte is captured by the first recognition molecule,
- wherein the light source is arranged for illumination of the membrane support layer, and the detector is positioned to detect light transmitted through the sensor chip.
7 more elements
A method of detecting a first analyte in a sample or of detecting a cell or exosome on which a first analyte and a second analyte are co-localized in a sample, the method comprising:
- a) An imaging system comprising:
- A light source;
- A detector;
- A sensor chip comprising:
- A conductive layer on a membrane support layer, wherein the conductive layer and the membrane support layer are disposed on a substrate, the substrate having a void formed therein in a region adjacent to a plurality of apertures to enable illumination of the membrane support layer to produce a surface plasmon resonance;
- The plurality of apertures extending through the conductive layer and the membrane support layer, wherein the plurality of apertures are arranged such that illumination of the membrane support layer produces the surface plasmon resonance;
- and
- A first recognition molecule that binds specifically to the first analyte, the first recognition molecule being immobilized onto a surface of the conductive layer;
- and
- A second recognition molecule that binds specifically to the first analyte or to the second analyte that is co-localized on a cell or exosome with the first analyte, wherein the second recognition molecule is coupled to a signal amplification moiety, and wherein the signal amplification moiety is capable of inducing the formation of an insoluble aggregate of increased optical density relative to that of the first analyte when the first analyte is captured by the first recognition molecule,
- Wherein the light source is arranged for illumination of the membrane support layer and the detector is positioned to detect light transmitted through the sensor chip;
- b) capturing the first analyte onto the surface of the conductive layer of the sensor chip;
- and
- c) detecting binding of the second recognition molecule to the captured first analyte on the surface of the conductive layer of the sensor chip, or to the second analyte co-localized on a cell or exosome with the captured first analyte, wherein an increased binding of the second recognition molecule as compared to a control sample indicates the presence of the first analyte in the sample or indicates the presence of a cell or exosome on which the first analyte and the second analyte are co-localized in the sample.
11 more elements
Dependencies are inferred from the claim text.