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US 12,523,593 B2Granted
Super resolution imaging of cell-cell interface
AssigneeMenachem Sherman
InventorEilon Sherman
Life of this patent
Estimating the term…
Abstract
The present technique provides a simple, cost-effective and robust method for re-alignment of interfaces between cell conjugates parallel to the coverslip. The technique is based on placement of two cell types, to subsequently form an interaction between the cell types, to opposing coverslips and then bringing them together before or during imaging, for either fixed or live cell imaging. Spacer particles having defined parameters control the z-separation and the relative lateral position of the opposing coverslips. We show that our method allows most types of super-resolution imaging.
Claims17
A method for use in imaging biological material, the method comprising:
- providing first sample comprising at least a first type of biological cells on a first transparent plate;
- providing second sample comprising at least a second type of biological cells on a second plate;
- placing said first transparent plate facing said second plate such that cells of the first sample are directed toward the cells of the second sample;
- providing said first and second plates facing each other in a microscopy system for collecting one or more microscopic images indicative of interface or interaction between at least one cell of the first sample and at least one cell of the second sample;
- providing one or more spacers between said first and second plates;
- said one or more spacers having an average diameter selected in accordance with sum of effective diameters of biological cells of at least the first and second types;
- wherein said one or more spacers have substantially spherical shape, said spacers being configured for use as bearings, thereby allowing parallel relative translational shift of the first and second plates;
- wherein the method further comprises varying relative translational location of said first and second plates to thereby electing specific cells of the first type interfacing or interacting with specific cells of the second type.
5 more elements
A system comprising:
- a kit comprising:
- a first transparent plate and a second plate;
- one or more spacers having selected diameter, said kit is adapted for use in imaging interface or interaction of biological cells;
- wherein diameter of said one or more spacers correspond to diameters of first and second types of biological cells for spacing the two plates for allowing (i) interface or interaction between cells of the first and second types of biological cells and (ii) movement of one of the plates with respect to the other in result to movement of the spacers;
- the system further comprising:
- at least one microscopic imaging unit comprising a sample mount;
- said sample mount being adapted for mounting an arrangement comprising said first transparent plate and said second plate separated by said one or more spacers;
- a translation module adapted for selectively shifting said second plate with respect to said first transparent plate, thereby enabling selective imaging of interface or interaction between regions of first and second samples positioned on the first and second plates respectively.
5 more elements
A method for use in imaging matter, the method comprising:
- providing first sample comprising first type of matter on a first transparent plate;
- providing second sample comprising second type of matter on a second plate;
- placing said first transparent plate facing said second plate such that matter of the first sample is directed toward the matter of the second sample;
- providing said first and second plates facing each other in a microscopy system for collecting one or more microscopic images indicative of interface or interaction between elements of the first and second types of matters;
- providing one or more spacers between said first and second plates;
- said one or more spacers having an average size selected in accordance with sum of the heights of the first and second types of matters, relative to their respective mounting plates;
- wherein said one or more spacers are mixed with one of said first and second matters, prior to their placement on the plate, the method further comprising fixing the mixture of the spacers and said first or second matters to the first or second plate.
4 more elements
A method for use in imaging matter, the method comprising:
- providing first sample comprising first type of matter on a first transparent plate;
- providing second sample comprising second type of matter on a second plate;
- placing said first transparent plate facing said second plate such that matter of the first sample is directed toward the matter of the second sample;
- providing said first and second plates facing each other in a microscopy system for collecting one or more microscopic images indicative of interface or interaction between elements of the first and second types of matters;
- wherein the interface of interaction is selected from any one of:
- 3D shape or arrangement of each of the interacting cells, dynamic changes in this shape before, along and after the interaction, the organization and shape of subcellular organelles and entities within the cells, organization of molecules within the cells and on their surface, changes in cell metabolism, cell mobility and arrest upon interaction.
3 more elements
Dependencies are inferred from the claim text.