Broad-spectrum nanoparticle enabled genomic detection
AssigneeThe Regents of the University of California
InventorsLiangfang Zhang, Wei Wang
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Abstract
Described are methods, compositions, and devices for early detection and prevention of diseases, including cancer, infectious diseases, and autoimmune diseases, using broad-spectrum enrichment technology and single cell sequencing technology. An example method for detecting circulating tumor cells (CTCs) using platelet membrane-coated nanoparticles (PNPs) and single cell RNA sequencing (scRNA-seq) technology is also provided.
Claims14
A method for enriching and genetically identifying target cells in a biological sample, comprising:
- enriching the target cells by contacting the biological sample with nanoparticles coated with a cell membrane that has a binding affinity with the target cells, wherein the nanoparticles consist of a polymeric particle core coated with the cell membrane that has a binding affinity with the target cells;
- and
- performing single cell nucleic acid sequencing on the enriched target cells.
A method for identifying a cancer type or subtype of circulating tumor cells (CTCs) in a biological sample, comprising:
- enriching the CTCs by contacting the biological sample with platelet membrane-coated nanoparticles that consist of a polymeric particle core coated with the platelet membrane;
- performing single cell nucleic acid sequencing (scRNA-seq) on the enriched CTCs;
- and
- determining the cancer type or subtype of the CTCs based on a comparison of the scRNA-seq and a plurality of genes whose expression profiles are associated with different types or subtypes of cancer.
Dependencies are inferred from the claim text.