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US 12,522,868 B2Granted
Polynucleotides, reagents, and methods for nucleic acid hybridization
AssigneeTwist Bioscience Corporation
InventorsRamsey Ibrahim Zeitoun, Siyuan Chen, Richard Gantt, Kristin D. Butcher, E. Hutson Chilton
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Abstract
Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.
Claims18
A method for nucleic acid hybridization, comprising:
- (a) contacting a first polynucleotide library with a second polynucleotide library, wherein at least one polynucleotide in the first polynucleotide library comprises a target sequence, at least one polynucleotide in the second polynucleotide library comprises a target binding sequence that is complementary to the target sequence, and wherein at least one and less than or equal to 90% of polynucleotides in the second polynucleotide library comprise a label;
- (b) enriching the at least one polynucleotide comprising the target sequence library to generate at least one enriched target polynucleotide;
- and
- (c) sequencing the at least one enriched target polynucleotide.
1 more element
Dependencies are inferred from the claim text.