Microbial production of cannabinoids
AssigneeAmyris, Inc.
InventorsVictor F. Holmes, John E. Hung, Irina A. Koryakina, Andrew P. Klein, Yi Xiong, William E. Draper, Hailley S. Warbington
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Abstract
The compositions and methods of the disclosure can be used to produce a cannabinoid in a host cell, such as a yeast cell. For example, the disclosure features host cells (e.g., yeast cells) modified to express one or more enzymes of a cannabinoid biosynthetic pathway, such as an acyl activating enzyme (AAE), a tetraketide synthase (TKS), a cannabigerolic acid synthase (CBGaS), and/or an olivetolic acid cyclase (OAC).
Claims18
A non-naturally occurring olivetolic acid cyclase (OAC) enzyme having an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:
- 44, wherein the OAC has one or more amino acid substitutions relative to the amino acid sequence of SEQ ID NO:
- 44 selected from A2S, L9I, K12S, E14S, V28L, T47R, Q48R, K49R, S87H, F88Y, and L92Y.
A non-naturally occurring OAC enzyme having an amino acid sequence that is at least 90% identical to the amino acid sequence of any one of SEQ ID NOs:
- 45-52.
Dependencies are inferred from the claim text.