Recombinant protein comprising multiple multi-peptide sets, pharmaceutical composition comprising the recombinant protein, and method for preparing the recombinant protein

A method for preparing a recombinant protein, comprising:

• (a) providing a human tyrosine hydroxylase of SEQ ID NO:
• 1, except that four substitutable regions correspond to amino acid residues 21-60, 68-95, 103-131, and 139-414;
• (b) substituting each region independently with at least one peptide having the amino acid sequence selected from the group consisting of peptide SEQ ID NO:
• 7-21 and the dipeptide KE, or the peptide SEQ ID NO:
• 24-39 and the tripeptide IPI;
• (c) transferring the post-replacement recombinant protein to a yeast expression system for fermentation;
• and
• (d) purifying with starch the post-fermentation recombinant protein to obtain the recombinant protein comprising the amino acid sequence of SEQ ID NO:
• 22.

A method for preparing a recombinant protein, comprising:

• (a) providing a human tyrosine hydroxylase of SEQ ID NO:
• 1, except that four substitutable regions correspond to amino acid residues 21-60, 68-95, 103-131, and 139-414;
• (b) substituting each region independently with at least one peptide having the amino acid sequence selected from the group consisting of peptide SEQ ID NO:
• 7-21 and the dipeptide KE, or the peptide SEQ ID NO:
• 24-39 and the tripeptide IPI;
• (c) transferring the post-replacement recombinant protein to a yeast expression system for fermentation;
• and
• (d) purifying with starch the post-fermentation recombinant protein to obtain the recombinant protein comprising the amino acid sequence of SEQ ID NO:
• 41.