Polymerase chain reaction apparatus and polymerase chain reaction method using same

A polymerase chain reaction apparatus comprising:

• a transparent photothermal substrate including a transparent plate on which an array of transparent nanopillars arranged to be spaced apart from each other is formed and plasmonic metal nanoislands positioned on surfaces including an upper surface and a side surface of the nanopillars;
• a light source which is positioned on a lower portion of the photothermal substrate and irradiates the plasmonic metal nanoislands with light;
• and
• a chamber containing a fluid heated by the transparent photothermal substrate,
• wherein
• the plasmonic metal nanoislands are positioned to be spaced apart from each other,
• the metal is gold,
• the transparent photothermal substrate is a glass substrate,
• an average diameter of the nanopillars is 50 to 1000 nm, and an aspect ratio obtained by dividing an average length of the nanopillars by the average diameter of the nanopillar is 0.1 to 10,
• a coverage, which is defined as a ratio of an area covered by the array of nanopillars to an area of the one side of the transparent plate, is 0.1 to 0.9,
• the plasmonic metal nanoislands positioned on the upper surface of the nanopillars have a shape corresponding to the upper surface of the nanopillars, and the plasmonic metal nanoislands positioned on the side surface of the nanopillars have an average diameter of 5 to 100 nm, and
• the photothermal substrate further includes a heat dissipation layer covering the nanopillars on which the plasmonic metal nanoislands are positioned.