Microfluidic device and method for use thereof for the separation, purification and concentration of components of fluid media

A microfluidic device, comprising:

• a structured component (1) formed as a flat body,
• a microfluidic channel system (2) formed in the structured component (1),
• at least one component (3), which is applied to a surface of a top side and/or a bottom side of the structured component (1) in order to seal the microfluidic channel system (2),
• at least one porous functional element (5),
• a fluidic reservoir (8), in which fluidic reagents are stored, and
• at least one fluidic interface (4.1, 4.2, 4.3), which is arranged at an external surface of the structured component (1) for the supply of media into the microfluidic channel system (2);
• and
• which includes reagents and/or dry reagents provided in at least one reaction chamber (6) and/or in at least one PCR chamber (20),
• wherein the at least one porous functional element (5) is in fluid communication with the at least one fluidic interface (4.1, 4.2, 4.3) and the fluidic reservoir (8) through the microfluidic channel system (2), and in response to the fluid communication, the at least one porous functional element (5) is configured to be flowed through in any order by the media supplied via the at least one fluidic interface (4.1, 4.2, 4.3) as well as by the fluidic reagents stored in the fluidic reservoir (8).