Composite product for the osteoarticular regeneration of cartilage lesion
AssigneesInstitut National De La Sante Et De La Recherhe Medicale (Inserm), Université De Stradbourg
InventorNadia Benkirane-Jessel
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Abstract
The present invention relates to a biomaterial comprising: —a membrane wound patch (a), made of a nanofibrous polymeric scaffold, —a hydrogel (b) including autologous or allogenic bone marrow-derived mesenchymal stem cells, and —a bone wound patch (c) being a nanofibrous scaffold made of polymers, wherein said scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch (c) further comprises a bone growth factor; wherein the hydrogel (b) is included between the membrane wound patch (a) and the bone wound patch (c).
Claims20
A biomaterial comprising:
- (a) a membrane wound patch made of a nanofibrous polymeric scaffold;
- (b) a hydrogel including stem cells, and
- (c) a bone wound patch of a nanofibrous patch scaffold made of polymers, wherein said nanofibrous patch scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch further comprises a bone growth factor;
- and
- wherein the hydrogel is located between the membrane wound patch and the bone wound patch.
A method for treating a cartilage lesion, said method comprising successively a step of applying a bone wound patch of a nanofibrous patch scaffold made of polymers, wherein said nanofibrous patch scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch further comprises a bone growth factor, a step of applying a hydrogel including stem cells and a step of applying a membrane wound patch made of a nanofibrous polymeric scaffold.
A method for treating bone and/or cartilage regeneration, said method comprising successively a step of applying a bone wound patch of a nanofibrous patch scaffold made of polymers, wherein said nanofibrous patch scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch further comprises a bone growth factor, a step of applying a hydrogel including stem cells and a step of applying a membrane wound patch made of a nanofibrous polymeric scaffold.
A method for treating a bone and/or cartilage defect, said method comprising successively a step of applying a bone wound patch of a nanofibrous patch scaffold made of polymers, wherein said nanofibrous patch scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch further comprises a bone growth factor a step of applying a hydrogel including stem cells and a step of applying a membrane wound patch made of a nanofibrous polymeric scaffold.
A method for treating and/or reducing the incidence of osteoarthritis, said method comprising a step of applying a biomaterial in a patient in need thereof, wherein said biomaterial comprises:
- (a) a membrane wound patch, made of a nanofibrous polymeric scaffold, wherein said polymeric scaffold has a surface being coated with either
- (ii) an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions including at least chondroitin sulfate and/or hyaluronic acid, and a layer of polycations, or
- (ii) a continuous coating comprising more than 15 layer pairs, each layer pair consisting of a layer of polyanions including at least chondroitin sulfate and/or hyaluronic acid, and a layer of polycations;
- (b) a bone wound patch of a nanofibrous patch scaffold made of polymers, wherein said nanofibrous patch scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch further comprises a bone growth factor;
- autologous or allogenic bone marrow-derived mesenchymal stem cells,
- a solution of hyaluronic acid and alginate, and
- a solution of calcium chloride or calcium phosphate.
A kit for treating and/or reducing an incidence of osteoarthritis, comprising:
- (a) a membrane wound patch, made of a nanofibrous polymeric scaffold;
- (b) a hydrogel including stem cells, and
- (c) a bone wound patch of a nanofibrous patch scaffold made of polymers, wherein said nanofibrous patch scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch further comprises a bone growth factor.
A kit for treating and/or reducing an incidence of osteoarthritis, comprising:
- a membrane wound patch, made of a nanofibrous polymeric scaffold, wherein said polymeric scaffold has a surface being coated with either
- (i) an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions including at least chondroitin sulfate and/or hyaluronic acid, and a layer of polycations, or
- (ii) a continuous coating comprising more than 15 layer pairs, each layer pair consisting of a layer of polyanions including at least chondroitin sulfate and/or hyaluronic acid, and a layer of polycations;
- a bone wound patch being a nanofibrous scaffold made of polymers, wherein said scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch further comprises a bone growth factor;
- autologous or allogenic bone marrow-derived mesenchymal stem cells,
- a solution of hyaluronic acid and alginate, and
- a solution of calcium chloride or calcium phosphate.
Dependencies are inferred from the claim text.