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US 12,521,450 B2Granted
DNA, expression vector, transcript, cell, pharmaceutical composition, and medicine
AssigneeHitachi, Ltd.
InventorAtsushi Okuma
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Abstract
An object of the invention is to provide a novel pharmaceutical composition. The pharmaceutical composition of the disclosure contains a DNA encoding a suicide gene having at least one intron sequence. The intron sequence has a donor sequence or an acceptor sequence to be used in a tumor cell with abnormal splicing not in a normal cell. In a transcript of the DNA, the suicide gene is expressed when the intron is abnormally spliced and the suicide gene is not expressed when the intron is not abnormally spliced.
Claims13
A DNA encoding a suicide gene comprising:
- at least one artificial intron comprising a nucleotide sequence having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8, SEQ ID NO 9, and SEQ ID NO 10,
- wherein the intron sequence has a donor sequence or an acceptor sequence, wherein the donor sequence or the acceptor sequence is used in abnormal splicing that occurs in a tumor cell and does not occur in a normal cell, and
- in a transcript of the DNA,
- the suicide gene is expressed when the abnormal splicing occurs, and
- the suicide gene is not expressed when the abnormal splicing does not occur.
2 more elements
Dependencies are inferred from the claim text.