Back to results
US 12,520,829 B2Granted
Transgenic cory
AssigneeGloFish, LLC
InventorsAlan Blake, Richard Crockett, Aidas Nasevicius
Life of this patent
Estimating the term…
Abstract
The present invention relates to transgenic ornamental fish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
Claims6
A transgenic Cory whose genome comprises:
- (a) a chromosomally integrated expression cassette encoding a red fluorescent protein, wherein the integrated expression cassette encoding the red fluorescent protein is designated as the “Red Cory 1 transformation event” comprised in the germ stem cells deposited as ATCC accession number PTA-127094, wherein the transgenic Cory exhibits red fluorescence,
- (b) a chromosomally integrated expression cassette encoding an orange fluorescent protein, wherein the integrated expression cassette encoding the orange fluorescent protein is designated as the “Orange Cory 1 transformation event” comprised in the germ stem cells deposited as ATCC accession number PTA-127095, wherein the transgenic Cory exhibits orange fluorescence, or
- (c) a chromosomally integrated expression cassette encoding a green fluorescent protein, wherein the integrated expression cassette encoding the green fluorescent protein is designated as the “Green Cory 1 transformation event” comprised in the germ stem cells deposited as ATCC accession number PTA-127096, wherein the transgenic Cory exhibits green fluorescence.
A method of producing a transgenic Cory comprising:
- (a) obtaining a transgenic Cory whose genome comprises:
- (i) a chromosomally integrated expression cassette encoding a red fluorescent protein, wherein the integrated expression cassette encoding the red fluorescent protein is designated as the “Red Cory 1 transformation event” comprised in the germ stem cells deposited as ATCC accession number 127094, wherein the transgenic Cory exhibits red fluorescence,
- (ii) a chromosomally integrated expression cassette encoding an orange fluorescent protein, wherein the integrated expression cassette encoding the orange fluorescent protein is designated as the “Orange Cory 1 transformation event” comprised in the germ stem cells deposited as ATCC accession number PTA-127095, wherein the transgenic Cory exhibits orange fluorescence, or
- (iii) a chromosomally integrated expression cassette encoding a green fluorescent protein, wherein the integrated expression cassette encoding the green fluorescent protein is designated as the “Green Cory 1 transformation event” comprised in the germ stem cells deposited as ATCC accession number PTA-127096, wherein the transgenic Cory exhibits green fluorescence, and
- (b) breeding the obtained transgenic Cory from step (a) with a second Cory to provide:
- (x) a transgenic Cory comprising the “Red Cory 1 transformation event” comprised in germ stem cell deposited as ATCC accession no. PTA-127094, wherein the transgenic Cory exhibits red fluorescence;
- (y) a transgenic Cory comprising the “Orange Cory 1 transformation event” comprised in germ stem cell deposited as ATCC accession no. PTA-127095, wherein the transgenic Cory exhibits orange fluorescence;
- or
- (z) a transgenic Cory comprising the “Green Cory 1 transformation event” comprised in germ stem cell deposited as ATCC accession no. PTA-127096,
- wherein the transgenic Cory exhibits green fluorescence.
7 more elements
Dependencies are inferred from the claim text.