Seal apparatus and method of using same

A single piece sterile-seal apparatus for sealing an opening of a structure having a thickness at the opening, the seal apparatus comprising:

• a first section disposed at a proximal end, the first section defining a collapsible cone-like portion including a plurality of radially spaced relief areas extending circumferentially about an outer surface thereof;
• a second section disposed at a distal end, the second section having a substantially cylindrical outer surface;
• an intermediate section, disposed between the first and second sections, the intermediate section including a radial groove projected axially into an inner wall of the second section and a recess in an inner wall of the first section under the collapsible cone-like portion;
• a bore extending through the first section, the second section, and the intermediate section,
• wherein the first section, the second section each contact the structure proximate to the opening, and the intermediate section contacts the opening to form a seal between a controlled environment and an environment external to the controlled environment when disposed within the opening of the structure.