Systems and methods for staining of biological samples
AssigneesVentana Medical Systems, Inc., Roche Diagnostics Operations, Inc.
InventorsAndreas Drechsler, Oliver Gutmann, Melis Hazar, Reto Huesser, Raymond T. Kozikowski, III, Daniel Mueller, Chris Steinert
Life of this patent
Estimating the term…
Abstract
A system and method for treatment of biological samples is disclosed. In some embodiments, an automated biological sample staining system (100), comprising at least one microfluidic reagent applicator (118); at least one bulk fluid applicator (116); at least one fluid aspirator; at least one sample substrate holder; at least one relative motion system; and a control system (102) that is programmed to execute at least one staining protocol on a sample mounted on a substrate that is held in the at least one sample substrate holder.
Claims11
A method for automated treatment of at least a portion of a paraffin-embedded sample held on a substrate, the method including:
- a. obtaining an image of the sample on the substrate, wherein the sample comprises a paraffin-embedded sample;
- b. automatically locating one or more positions of the sample on the substrate, wherein the automatically locating of the one or more positions of the sample on the substrate comprises automatically detecting a portion of a paraffin section that contains the sample;
- and
- c. forming one or more wells in the paraffin of the paraffin-embedded sample at the one or more automatically located positions, wherein the one or more wells are formed by (i) applying a deparaffinization fluid to the one or more automatically located positions, and (ii) removing the applied deparaffinization fluid from the one or more automatically located positions;
- and
- d. dispensing one or more additional fluids to the formed one or more wells after the deparaffinization fluid is removed.
The method of 9, wherein the first and second needle are rotated around a common axis.
Dependencies are inferred from the claim text.