Catalytic signal enhancement for lateral flow immunoassays
AssigneeThe Government of the United States of America, as represented by the Secretary of the Navy
InventorDavid A. Kidwell
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Abstract
The present invention provides a method for increasing the sensitivity of LFIAs by using palladium nanoparticles, selecting appropriate dye chemistries, and improving the timing of the development chemistry. In the presence of a palladium nanoparticle, three reagents interact with a catalytic label to form a colored dye. The three reagents include a hydrogen peroxide source, a color developer (a substituted para-phenylenediamine), and a color coupler (e.g. a napthol or a phenol). The timing of the development chemistry is improved by any combination of using a reducing agent, delaying hydrogen peroxide application by diffusion, using dissolving materials as a time delay, using serpentine flow, and separating the color coupler and the color developer on the strip.
Claims2
A method for improving a catalytic signal for a lateral flow immunoassay, comprising:
- providing a lateral flow immunoassay comprising
- a source of hydrogen peroxide, wherein the source of hydrogen peroxide comprises sodium percarbonate;
- a substituted para-phenylenediamine;
- a phenol or napthol;
- and
- palladium nanoparticles;
- and
- interacting the substituted para-phenylenediamine, the phenol or napthol, and the hydrogen peroxide with the palladium nanoparticles to form a dye that is a visual label for the lateral flow immunoassay.
A method for improving a catalytic signal for a lateral flow immunoassay, comprising:
- providing a lateral flow immunoassay comprising
- a source of hydrogen peroxide;
- a substituted para-phenylenediamine, wherein the substituted para-phenylenediamine comprises N-ethyl-N-(2-hydroxyethyl)-2-methyl-1,4-phenylenediamine (HEPDA) or N,(2-((4-amino-3-methylphenyl)(ethyl)amino)ethylmethanesulfonamide (MSPDA);
- a phenol or napthol;
- and
- palladium nanoparticles;
- and
- interacting the substituted para-phenylenediamine, the phenol or napthol, and the hydrogen peroxide with the palladium nanoparticles to form a dye that is a visual label for the lateral flow immunoassay.
Dependencies are inferred from the claim text.