Layered detection device

A method of forming a biomarker detection device, the method comprising:

• providing a detection layer configured to detect one or more biomarkers in a biological sample, the detection layer comprising two or more detection zones;
• providing a transfer layer in direct contact with a first surface of the detection layer;
• providing two or more observation windows on a second surface of the detection layer opposite the transfer layer, each observation window corresponding to a respective detection zone;
• providing an adhesive layer;
• and
• bonding the detection layer and transfer layer together and wherein there is no adhesive between the transfer layer and the detection layer in the region in register with the observation window;
• wherein one or more gaps are provided between adjacent observation windows;
• wherein the adhesive layer is bonded to the transfer layer and the detection layer and the transfer layer is bonded to the detection layer in the one or more gaps.