Glucose-6-phosphate dehydrogenase mutant and use thereof in preparing detection reagent

The detection kit according to claim 4, comprising:

• a first reagent comprising:
• 10 mM to 500 mM buffer,
• 5 mM to 25 mM substrate,
• 0.01 μg/L to 1 mg/L anti-hapten antibody,
• 10 mM to 300 mM NaCl,
• 0.1 g/L to 5 g/L stabilizer,
• 0.1 g/L to 5 g/L surfactant,
• 0.1 g/L to 5 g/L preservative;
• a second reagent comprising:
• 10 mM to 500 mM buffer,
• 0.01 μg/L to 1 mg/L conjugate of claim 2,
• 0.1 g/L to 5 g/L stabilizer,
• 0.1 g/L to 5 g/L surfactant,
• 0.1 g/L to 5 g/L preservative;
• the buffer is selected from one or a combination of the following:
• tromethamine buffer, phosphate buffer, Tris-HCl buffer, citric acid-sodium citrate buffer, barbiturate buffer, glycine buffer, borate buffer and trihydroxymethyl methane buffer;
• the buffer of the first reagent and the buffer of the second reagent are the same or different;
• the concentration of the buffer is 10 mmol/L to 500 mmol/L;
• the pH of the buffer is 6.5 to 8.0;
• the stabilizer is selected from the following one or a combination of:
• bovine serum albumin, trehalose, glycerol, sucrose, mannitol, glycine, arginine, polyethylene glycol 6000 and polyethylene glycol 8000;
• the surfactant is selected from the following one or a combination of:
• Brij23, Brij35, Triton X-100, Triton X-405, Tween20, Tween30, Tween80, coconut oil fatty acid diethanolamide and AEO7;
• the preservative is selected from the following one or a combination of:
• azide, MIT, PC biological preservative and thimerosal;
• the azide is selected from the group consisting of:
• sodium azide, lithium azide and PC-300;
• the substrate comprises:
• glucose 6-phosphate and β-nicotinamide adenine dinucleotide.