Primary gastrointestinal stromal tumor cell culture medium, culture method and application thereof
AssigneePrecedo Pharmaceuticals Co., Ltd
InventorsQingsong Liu, Feiyang Liu, Xiaoyu Li, Husheng Mei, Wenchao Wang, Tao Ren, Li Wang
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Abstract
The present invention provides a cell culture medium for culturing primary gastrointestinal stromal tumor cells, comprising gastrin, N2, insulin, a receptor tyrosine kinase ligand, and a Rock kinase inhibitor. The present invention further provides a method for culturing gastrointestinal stromal tumor cells by using the cell culture medium, and an application and a method of an expanded cell population, which is obtained by using the method, in efficacy evaluation or screening.
Claims15
A cell culture medium for culturing primary gastrointestinal stromal tumor cells, wherein
- the cell culture medium comprises gastrin, N2, insulin, platelet-derived growth factor AA, stem cell factor, basic fibroblast growth factor, and a Rock kinase inhibitor.
Dependencies are inferred from the claim text.