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US 12,517,020 B2Granted
3D-printed microfluidic devices for solid-phase extraction and fluorescent labeling of peptides and proteins
AssigneeBrigham Young University
InventorsAdam T. Woolley, Anna V. Bickham, Gregory P. Nordin
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Abstract
Miniaturized solid-phase extraction (SPE) systems and processes are disclosed. The systems and methods enable preconcentration methods for sample preparation that can be performed on a variety of specimens. The miniaturization of SPE system is accomplished with 3D-printed microfluidic device for fast and simple extraction of analytes.
Claims18
A method of identifying a presence or an amount of a biomolecule, comprising:
- providing a porous, monolith substrate from a 3D printer, the monolith substrate further comprising:
- one or more monolith columns fluidly connected to one or more vacuum-attachment ports, a side, a top, and a bottom;
- loading an analyte containing sample into the one or more monolith columns;
- loading a solvent into the one or more monolith columns, thereby eluting the biomolecule from the one or more monolith columns;
- and
- identifying the presence or the amount of the biomolecule from the analyte containing sample,
- wherein the one or more monolith columns are made from polymerized lauryl methacrylate monomer, the one or more vacuum-attachment ports comprise one or more horizontal vacuum-attachment ports located horizontally in relation to the top and the bottom of the monolith substrate, the one or more horizontal vacuum-attachment ports comprises an end that protrudes from the side of the monolith substrate, and the biomolecule is a protein.
4 more elements
A system for solid-phase extraction and on-chip fluorescent labeling, comprising:
- an extraction chip with a porous, monolith substrate from a 3D printer, the monolith substrate further comprising:
- one or more monolith columns fluidly connected to one or more vacuum-attachment ports, a side, a top, and a bottom;
- a buffer solution;
- a loading solution;
- a fluorescent labeling solution;
- and
- a first elution solution,
- wherein the one or more monolith columns are made from polymerized lauryl methacrylate monomer, the one or more vacuum-attachment ports comprise one or more horizontal vacuum-attachment ports located horizontally in relation to the top and the bottom of the monolith substrate, the one or more horizontal vacuum-attachment ports comprises an end that protrudes from the side of the monolith substrate.
5 more elements
A method of concentrating a target analyte, comprising:
- providing a porous, monolith substrate from a 3D printer, the monolith substrate further comprising:
- one or more monolith columns fluidly connected to one or more vacuum-attachment ports, a side, a top, and a bottom;
- loading an analyte containing sample into the one or more monolith columns in which the target analyte adheres to the one or more monolith columns;
- loading a buffer solution through the one or more monolith columns;
- and
- eluting the target analyte from the one or more monolith columns,
- wherein the one or more monolith columns are made from polymerized lauryl methacrylate monomer, the one or more vacuum-attachment ports comprise one or more horizontal vacuum-attachment ports located horizontally in relation to the top and the bottom of the monolith substrate, and the one or more horizontal vacuum-attachment ports comprises an end that protrudes from the side of the monolith substrate.
4 more elements
A method of concentrating and fluorescently labeling a target analyte, comprising:
- providing a porous, monolith substrate from a 3D printer, the monolith substrate further comprising:
- one or more monolith columns fluidly connected to one or more vacuum-attachment ports, a side, a top, and a bottom;
- loading an analyte containing sample into the one or more monolith columns in which the target analyte adheres to the one or more monolith columns;
- loading a fluorescent labeling solution through the one or more monolith columns, thereby generating a labeled target analyte;
- loading a buffer solution through the one or more monolith columns;
- and
- eluting the labeled target analyte from the one or more monolith columns, wherein the one or more monolith columns are made from polymerized lauryl methacrylate monomer, the one or more vacuum-attachment ports comprise one or more horizontal vacuum-attachment ports located horizontally in relation to the top and the bottom of the monolith substrate, and the one or more horizontal vacuum-attachment ports comprises an end that protrudes from the side of the monolith substrate.
4 more elements
Dependencies are inferred from the claim text.