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US 12,516,361 B2Granted
Genetically engineered eukaryotic cells producing sialylated glycoproteins
AssigneesUniversiteit Gent, Vib Vzw
InventorsNico Callewaert, Wander Van Breedam, Francis Santens
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Abstract
The present application provides glycosylation engineered higher eukaryotic cells which produce homogeneous forms of sialylated glycans present on a recombinant glycoprotein. In particular, the glycosylation engineered higher eukaryotic cells produce homogeneous forms of small N-glycan structures which carry terminal sialic acid residues. In addition, the invention provides glycan-conjugates based on specific coupling with sialic acid residues present on the recombinant glycoproteins.
Claims8
A mammalian cell comprising:
- a first exogenous nucleic acid sequence encoding an endoglucosaminidase enzyme, wherein the endoglucosaminidase enzyme is operably linked to a secretion signal or is operably linked to an ER or Golgi localization signal;
- a second nucleic acid sequence encoding a mutant UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase which is insensitive to CMP-Neu5Ac feedback inhibition;
- and
- a third exogenous nucleic acid sequence encoding a Fc containing molecule;
- wherein the Fc containing molecules produced by the cell have at least a 30% increase in sialyl-LacNAc N-glycans as compared to otherwise identical cells lacking the second nucleic acid.
2 more elements
Dependencies are inferred from the claim text.