Compositions and methods useful for the regulation of abiotic stress responses in higher plants
AssigneeBoyce Thompson Institute for Plant Research, Inc.
InventorsDavid Stern, Coralie Evelyn Salesse-Smith, Leila Feiz
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Abstract
Compositions and methods for creating plants exhibiting enhanced resistance to abiotic stresses, especially cold stress are disclosed.
Claims11
A transgenic C4 plant comprising heterologous, recombinant nucleic acids expressing rubisco small subunit (SS), rubisco large subunit (LS), and ribulose-1,5-Bis-Phosphate Carboxylase/Oxygenase Accumulation Factor 2 (RAF2), expression of said SS, LS and RAF2 increasing holoenzyme accumulation, said plant exhibiting increased rubisco content, increased photosynthetic rate, increased biomass and enhanced resistance to abiotic stress when compared to plants lacking said heterologous nucleic acids.
A stacked transgenic maize plant line comprising heterologous, recombinant nucleic acids expressing rubisco small subunit (SS), rubisco large subunit (LS), ribulose-1,5-Bis-Phosphate Carboxylase/Oxygenase Accumulation Factor 2 (RAF2), and optionally bundle sheath defective 2 (BSD2), wherein said heterologous recombinant nucleic acids are stacked in combinations comprising:
- i) RAF2, SS, and LS;
- or
- ii) RAF2, SS, LS, BSD2;
- said transgenic maize plant lines exhibiting at least one of increased rubisco content, increased photosynthetic rate, increased biomass and enhanced resistance to abiotic stress when compared to plants lacking said stacked heterologous nucleic acids.
Dependencies are inferred from the claim text.