3′-UTR sequences for stabilization of RNA
AssigneesBioNTech SE, TRON-Translationale Onkologie an der Universitätsmedizin der Johannes Gutenberg-Universität Mainz gGmbH
InventorsAlexandra Orlandini Von Niessen, Stephanie Fesser, Britta Vallazza, Tim Beissert, Andreas Kuhn, Ugur Sahin, Marco Alexander Poleganov
Life of this patent
Estimating the term…
Abstract
The present invention relates to stabilization of RNA, in particular mRNA, and an increase in mRNA translation. The present invention particularly relates to a modification of RNA, in particular in vitro-transcribed RNA, resulting in increased transcript stability and/or translation efficiency. According to the invention, it was demonstrated that certain sequences in the 3′-untranslated region (UTR) of an RNA molecule improve stability and translation efficiency.
Claims20
A method of expressing a polypeptide in a subject by administering an RNA molecule to the subject, wherein said RNA molecule comprises, in the 5′→3′ direction:
- (a) a 5′-untranslated region;
- (b) a nucleic acid sequence coding for a peptide or protein;
- and
- (c) a 3′-untranslated region which is not naturally linked to the nucleic acid sequence of (b),
- said 3′-untranslated region comprising:
- a nucleic acid sequence of the 3′-untranslated region of Amino-Terminal Enhancer of Split (AES), which comprises (A) a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 86 to 89, (B) a fragment comprising at least 80% of any one of SEQ ID NOs:
- 86 to 89, or (C) a variant comprising a nucleic acid sequence that is at least 90% identical to the nucleic acid sequence (A) or the fragment (B).
Dependencies are inferred from the claim text.