Methods for isolating DNA from a blood sample
AssigneeBiocaptiva Limited
InventorsMark Bradley, Matthew Owens
Life of this patent
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Abstract
The present invention relates to methods for isolating nucleic acids present in a sample, in particular cell-free DNA (cfDNA) from a blood sample and polymers, substrates and kits for the method. Polymers with characteristics suitable to bind such nucleic acids are provided.
Claims20
A nucleobase containing polymer comprising a polymer backbone with at least a portion of side chains being nucleobase side chains, wherein the polymer further comprises other positively charged moieties at physiological pH adapted to electrostatically interact with nucleic acids to be bound, and/or the polymer is adapted to interact with the nucleic acids to be bound via hydrogen bonding, and wherein the polymer comprises:
- (i) one or more monomers selected from the group consisting of:
- 2-methoxyethyl acrylate (MEA), 2-methoxyethyl methacrylate (MEMA), diethylamino ethylacrylate (DEAEA), diethylamino ethyl methacrylate (DEAEMA), hydroxyethyl acrylate, hydroxyethyl methacrylate, methyl methacrylate, methyl acrylate, styrene, methyl styrene, glycidyl acrylate, glycidyl methacrylate, N-vinylacetamide, 2-methyl-2-nitropropyl methacrylate, acrylic acid, methacrylic acid, 2-[[(butylamino)carbonyl]oxy] ethylacrylate, dimethylamino ethyl acrylate, dimethylamino ethyl methacrylate, mono-2-(acryloyloxy) ethyl succinate, poly(ethylene glycol) methyl ether acrylate (PEGA), and poly(ethylene glycol) methyl ether methacrylate (PEGMA);
- and (ii) one or more nucleobase-containing monomers.
A nucleobase containing polymer comprising a polymer backbone with at least a proportion of side chains being nucleobase side chains, wherein the polymer further comprises other positively charged moieties at physiological pH adapted to electrostatically interact with nucleic acids to be bound, and/or the polymer is adapted to interact with the nucleic acids to be bound via hydrogen bonding, and wherein the polymer comprises:
- (i) one or more monomers selected from the group consisting of:
- 2-methoxyethyl acrylate (MEA), 2-methoxyethyl methacrylate (MEMA), diethylamino ethylacrylate (DEAEA), diethylamino ethyl methacrylate (DEAEMA), hydroxyethyl acrylate, hydroxyethyl methacrylate, methyl methacrylate, methyl acrylate, styrene, methyl styrene, glycidyl acrylate, glycidyl methacrylate, N-vinylacetamide, 2-methyl-2-nitropropyl methacrylate, acrylic acid, methacrylic acid, 2-[(butylamino)carbonyl]oxy] ethylacrylate, dimethylamino ethyl acrylate, dimethylamino ethyl methacrylate, mono-2-(acryloyloxy) ethyl succinate, poly(ethylene glycol) methyl ether acrylate (PEGA), and poly(ethylene glycol) methyl ether methacrylate (PEGMA);
- and (ii) one or more other (meth)acrylate-based monomers with nucleobase side chains.
A nucleobase containing polymer comprising a polymer backbone with at least a proportion of side chains being nucleobase side chains, wherein the polymer further comprises other positively charged moieties at physiological pH adapted to electrostatically interact with nucleic acids to be bound, and/or the polymer is adapted to interact with the nucleic acids to be bound via hydrogen bonding, and wherein the polymer is synthesized from:
- (i) one or more (meth)acrylate-based monomers, and (ii) one or more nucleobase-containing monomers.
Dependencies are inferred from the claim text.