Beta-glycosidase SS-BGL mutant for modifying ginsenoside and application thereof
OwnerWeina Li, Daidi Faninventors
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Abstract
A β-glycosidase SS-BGL mutant for modifying ginsenoside and an application thereof are provided, which relate to the field of genetic engineering technologies. The β-glycosidase SS-BGL mutant is a mutant mutating asparagines at 128th position and 302th position of the amino acid sequence as shown in SEQ ID NO: 1 of β-glycosidase SS-BGL into aspartic acids respectively. The β-glucosidase SS-BGL mutant improves the thermal stability of the natural β-glucosidase SS-BGL at extremely high temperature, and is more conducive to the application of SS-BGL in ginsenoside preparation.
Claims7
A Sulfolobus solfataricus-β-glycosidase (SS-BGL) mutant for modifying ginsenoside, wherein the SS-BGL mutant is a mutant with the amino acid sequence of SEQ ID NO:
- 18;
- wherein the SS-BGL mutant is formed by mutating asparagines at 128th position and 302th position of the amino acid sequence of SEQ ID NO:
- 1 of SS-BGL into aspartic acids respectively.
An SS-BGL mutant for modifying ginsenoside, wherein the amino acid sequence of the SS-BGL mutant is SEQ ID NO:
- 18.
Dependencies are inferred from the claim text.