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US 12,516,281 B2Granted
Cell isolation device and method
AssigneeCutiss AG
InventorsClaude Nicolas Holenstein, Vincent Ronfard, Anna-Lena Dittrich, Reto Frei, Christian Stefan Wullschleger, Jascha Eisenberg, Sebastian Wollmann
Life of this patent
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Abstract
The present invention relates to a device and method for automatically isolating viable cells from connective, epithelial or other tissue, and, if the tissue is multilayered, for automatically separating one tissue layer from another.
Claims21
An automatically operable cell isolation device, comprising:
- a) a liquid distribution unit that includes a plurality of separate liquid containers;
- b) a biopsy kit by which a sample of tissue is extractable;
- c) a main disposable unit configured to cooperate with one or more process containers and with a valve array;
- d) one or more final product bags for receiving isolated cells,
- wherein a first portion of said valve array interfaces between said biopsy kit and one of said process containers, a second portion of said valve array interfaces between said liquid distribution unit and said one or more process containers, and a third portion of said valve array interfaces between said one or more process containers and said one or more final product bags, wherein said valve array is configurable to effect a desired cell isolation procedure;
- e) comprising a valve controller for facilitating selective actuation of each valve of the valve array to achieve a desired valve configuration;
- f) means for generating fluid flow and tissue transfer between the biopsy kit and the one or more process containers or between the one or more process containers and the one or more final product bags in conjunction with the desired valve configuration;
- and
- g) a sequencer module in data communication with the valve controller and the fluid generating means for facilitating selective actuation of each valve of the valve array and of the fluid generating means in each step of a sequence of the desired cell isolation procedure.
6 more elements
An automatically operable cell isolation device, comprising:
- a) a liquid distribution unit that includes a plurality of separate liquid containers;
- b) a biopsy kit by which a sample of tissue is extractable;
- c) a main disposable unit configured to cooperate with one or more process containers and with a valve array;
- d) one or more final product bags for receiving isolated cells,
- wherein a first portion of said valve array interfaces between said biopsy kit and one of said process containers, a second portion of said valve array interfaces between said liquid distribution unit and said one or more process containers, and a third portion of said valve array interfaces between said one or more process containers and said one or more final product bags, wherein said valve array is configurable to effect a desired cell isolation procedure;
- wherein the main disposable unit comprises a disposable tube system for being in fluid communication with each of the first, second and third portions of the valve array through which liquid discharged from the liquid distribution unit is selectively flowable;
- and
- e) a housing in which are mounted a plurality of electrical or electronic components including the valve array which are configured to effect the desired cell isolation procedure;
- wherein the main disposable unit is a carrier in which the tube system is encapsulated and which is adapted to be securely coupled in error-free fashion with a front panel of the housing such that the tube system is set in fluid communication with each of the first, second and third portions of the valve array.
6 more elements
A method for automatically performing a cell isolation procedure, comprising the steps of:
- a) providing a housing in which are mounted a plurality of electrical or electronic components configured to effect a desired cell isolation procedure;
- b) securely coupling a carrier, in which a tube system through which one or more isolation agents for isolating extracted tissue is flowable is encapsulated, with the housing, such that the tube system is selectively set in communication by error-free fashion with the plurality of electrical or electronic components after the carrier is coupled with the housing;
- c) introducing a tissue sample into a tube of the tube system;
- d) selecting a desired protocol for performing a cell isolation procedure by means of one of the electronic components;
- and
- e) performing the cell isolation procedure by sequentially operating the plurality of electrical or electronic components until the one or more isolation agents flows through the tube system and sufficiently acts on the introduced extracted tissue to isolate a cell population residing in the introduced extracted tissue to a single cell level.
3 more elements
Dependencies are inferred from the claim text.