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US 12,516,120 B2Granted
Methods and systems for T cell expansion
AssigneeGeorgia Tech Research Corporation
InventorsKrishnendu Roy, Nate Dwarshuis, Ranjna Madan-Lala, Kyung-Ho Roh, Hannah Kathryn Wilson
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Abstract
The present disclosure provides a system for mimicking the secondary lymphoid organs where suspension cells (e.g., T cells) are expanded; methods el expanding activating, and transfecting the suspension cells in the synthetic, microenvironment, and suspension cells produced by such systems and methods.
Claims21
A method of expanding, activating, and/or transfecting suspension cells, the method comprising:
- obtaining a blood sample from a patient;
- isolating suspension cells from the blood sample;
- introducing the suspension cells to a bioreactor comprising a suspended macroporous microcarrier;
- activating the suspension cells;
- expanding the suspension cells;
- optionally transfecting the suspension cells;
- preparing the suspension cells for transfusion into the patient;
- and
- transfusing the suspension cells into the patient;
- wherein the macroporous microcarrier comprises gelatin or other extracellular matrix components and is functionalized with antibodies that are specific for activation of the suspension cells;
- and
- wherein the suspension cells are T cells.
9 more elements
Dependencies are inferred from the claim text.