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US 12,515,445 B2Granted
Polymer sheets for sequencing applications
AssigneeIllumina, Inc.
InventorsBala Murali Venkatesan, Kenny Chen, Steven M. Barnard
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Abstract
Embodiments of the present application relate to patterned polymer sheets and processes to prepare the same for sequencing applications. In particular, flexible micro- and nano-patterned polymer sheets are prepared and used as a template surface in sequencing reaction and new polish-free methods of forming isolated hydrogel plugs in nanowells are described.
Claims19
A process for preparing a patterned polymer sheet for sequencing applications, comprising:
- providing a substrate with a patterned surface, wherein said surface comprises a plurality of micro-scale or nano-scale patterned wells;
- depositing a polymer composition onto the patterned surface to form a first polymer layer, wherein the polymer composition fills the micro-scale or nano-scale patterned wells;
- dehydrating the first polymer layer to form a dehydrated first polymer sheet;
- and
- separating the dehydrated first polymer sheet from the patterned surface, wherein the polymer composition is filled in the micro-scale or nano-scale patterned wells of the patterned surface is isolated in micro-scale or nano-scale patterned wells after separation of the dehydrated first polymer sheet.
2 more elements
Dependencies are inferred from the claim text.