Altering microbial populations and modifying microbiota
AssigneeSNIPR Technologies Limited
InventorsJasper Clube, Morten Sommer, Christian Grøndahl, Eric Van Der Helm, Ruben Vazquez-Uribe
Life of this patent
Estimating the term…
Abstract
The invention relates to methods, uses, systems, arrays, engineered nucleotide sequences and vectors for inhibiting bacterial population growth or for altering the relative ratio of sub-populations of first and second bacteria in a mixed population of bacteria. The invention is particularly useful, for example, for treatment of microbes such as for environmental, medical, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system.
Claims23
A method for modifying a mixed population of bacteria, wherein the mixed population of bacteria comprises a first bacterial sub-population and a second bacterial sub-population, wherein the first bacterial sub-population comprises a first bacterial species and the second bacterial sub-population comprises a population of host cells of a second bacterial species, wherein the second bacterial species is a different species than the first bacterial species, the method comprising:
- producing a crRNA from an engineered nucleic acid in the host cells, wherein the crRNA hybridizes to a target sequence in the host cells and guides a Cas to modify the target sequence in the host cells,
- wherein the first bacterial species is a gram positive species and the second bacterial species is a gram positive species,
- wherein the growth of the first bacterial species in the mixed population is not inhibited, and wherein the host cells are killed or growth of the host cells is reduced.
A method for modifying a mixed population of bacteria, wherein the mixed population of bacteria comprises a first bacterial sub-population and a second bacterial sub-population, wherein the first bacterial sub-population comprises a first bacterial species and the second bacterial sub-population comprises a population of host cells of a second bacterial species, wherein the second bacterial species is a different species than the first bacterial species, the method comprising:
- producing a crRNA from an engineered nucleic acid in the host cells, wherein the crRNA hybridizes to a target sequence in the host cells and guides a Cas to modify the target sequence in the host cells,
- wherein the first bacterial species has a 16s ribosomal RNA-encoding DNA sequence that is at least about 80% identical to a 16s ribosomal RNA-encoding DNA sequence of the second bacterial species,
- wherein the growth of the first bacterial species in the mixed population is not inhibited, and wherein the host cells are killed or growth of the host cells is reduced.
A method for modifying a mixed population of bacteria, wherein the mixed population of bacteria comprises a first bacterial sub-population and a second bacterial sub-population, wherein the first bacterial sub-population comprises a first bacterial species and the second bacterial sub-population comprises a population of host cells of a second bacterial species, wherein the second bacterial species is a different species than the first bacterial species, the method comprising:
- producing a crRNA from an engineered nucleic acid in the host cells, wherein the crRNA hybridizes to a target sequence in the host cells and guides a Cas in the host cells to modify the target sequence in the host cells,
- wherein the first bacterial species is a Firmicutes and the second bacterial species is a Firmicutes,
- wherein the growth of the first bacterial species in the mixed population is not inhibited, and wherein the host cells are killed or growth of the host cells is reduced.
Dependencies are inferred from the claim text.