Synergist therapy for enhanced drug delivery: magnetic field facilitated nanoparticle microporation
AssigneeKansas State University Research Foundation
InventorsViktor Chikan, Ryan Rafferty
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Abstract
Methods for enhancing intracellular uptake of active agents by inducing temporary pore formation in a cell membrane are described. The methods generally comprise introducing nanoparticles to a cell in vivo, ex vivo, or in cell culture to magnetic nanoparticles that are taken up into the cell interior. The methods further comprise introducing active agents to the cell. A magnetic field is applied to the cell for targeted excitation of the internalized magnetic nanoparticles to induce temporary pore formation in the cell membrane, such that the active agent is taken up in an increased amount and/or at an increased rate by the cell.
Claims17
A method of enhancing intracellular uptake of active agents into the interior of a cell by inducing temporary pore formation in a cell membrane, said method comprising:
- targeting a cell in vivo, ex vivo, or in cell culture with magnetic nanoparticles, wherein said magnetic nanoparticles are taken up by said cell into the interior of the cell to yield internalized magnetic nanoparticles;
- separately presenting one or more active agents to said cell, before, after, or simultaneously with said magnetic nanoparticles;
- and
- applying an inhomogeneous magnetic field to said cell for targeted excitation of said internalized magnetic nanoparticles to induce said temporary pore formation in said cell membrane to yield pores in said cell membrane and an altered cell having temporarily increased permeability, wherein after said applying step said one or more active agents are taken up in an increased amount and/or at an increased rate by said altered cell having temporarily increased permeability as compared to an untreated cell.
Dependencies are inferred from the claim text.