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US 12,514,253 B2Granted
Method for inducing RNA silencing
AssigneeAjinomoto Co., Inc.
InventorsShuhei Hashiro, Hisashi Yasueda, Mayu Nakano, Teruyuki Niimi, Haruka Kawaguchi
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Abstract
A method for efficiently inducing RNA silencing in a target organism is provided. RNA silencing is induced in a target organism by allowing the target organism to ingest cells of a microorganism, wherein the microorganism is able to produce RNA that induces RNA silencing after treating the cells with an organic solvent.
Claims8
A method for inducing RNA silencing in a target organism, the method comprising:
- allowing the target organism to ingest dead cells of a microorganism,
- wherein the microorganism is a coryneform bacterium,
- wherein the microorganism expresses RNA,
- wherein the RNA induces RNA silencing in the target organism,
- wherein the dead cells have been subjected to a treatment with an organic solvent,
- wherein the dead cells comprise said RNA,
- wherein the organic solvent is ethanol or methanol, and the concentration of the organic solvent in the treatment is 30% (v/v) or higher when the organic solvent is ethanol and 50% (v/v) or higher when the organic solvent is methanol.
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Dependencies are inferred from the claim text.