Systems and related pump manifold assemblies

An apparatus, comprising:

• a flow cell interface adapted to be coupled to a flow cell having a plurality of channels, a front, and a rear;
• and
• a pump manifold assembly carrying a plurality of pump valves and a plurality of pumps and comprising a plurality of pump-channel fluidic lines, a plurality of pump fluidic lines, and a shared fluidic line, the pump valves and the pumps are operable to individually control fluid flow through each channel of the plurality of channels of the flow cell via the corresponding pump-channel fluidic lines, each pump valve being coupled to a corresponding pump-channel fluidic line, a corresponding pump fluidic line, and the shared fluidic line and being movable between a first position fluidically coupling a corresponding channel of the plurality of channels, a corresponding pump-channel fluidic line, and a corresponding pump fluidic line and a second position fluidically coupling a corresponding pump fluidic line, the shared fluidic line, and a waste reservoir, each pump coupled to a corresponding pump fluidic line;
• a reagent reservoir positioned upstream of the flow cell interface and fluidly connected to the front of the flow cell interface;
• and
• a sample cartridge comprising a plurality of sample wells and positioned downstream of the flow cell interface, fluidically between the flow cell interface and the pump manifold assembly, and fluidly connected to the rear of the flow cell interface;
• wherein when the flow cell interface is coupled to the flow cell, the front of the flow cell is fluidly connected to the reagent reservoir, and the rear of the flow cell is fluidly connected to the sample cartridge, the plurality of pumps is operable to load one or more of the channels of the plurality of channels of the flow cell with a sample of interest from the sample cartridge by flowing the sample of interest in a first direction into the one or more channels of the flow cell, and the plurality of pumps is operable to flow reagent from the reagent reservoir through the channels of the flow cell in a second direction, opposite the first direction, and
• wherein the waste reservoir is fluidly connected to the front of the flow cell by an auxiliary waste fluidic line and to the rear of the flow cell by a primary waste fluidic line.