Method and composition for rapid detection of protein soils

A method for detecting protein on a surface, the method comprising:

• (a) applying a composition to the surface, wherein the composition comprises:
• i. a first part comprising 0.01 to 0.18 wt-% hydrated copper (II) sulfate;
• and
• ii. a second part comprising 0.2 to 3.0 wt-% of bicinchoninic acid to produce a visible color reaction when contacted with protein if present on the surface;
• wherein the hydrated copper (II) sulfate and the bicinchoninic acid are applied at a ratio of 5:1 to a ratio of 1:7,
• (b) observing any color reaction on the surface at room temperature within 30 seconds from applying the composition, wherein the color reaction indicates the presence of protein on the surface and the absence of the color reaction indicates the absence of protein on the surface,
• (c) recording the presence or absence of a color reaction, and
• (d) upon detection of the color reaction, training cleaning personnel on at least one of cleaning chemicals, cleaning tools, and cleaning techniques.