Method and apparatus for analyzing flow matrix data, and computer device

An apparatus for analyzing flow matrix data, comprising:

• a laser that emits a beam on microsphere particles;
• a data acquisition module configured to acquire and sample a forward-scattered light pulse signal outputted by a flow matrix analyzer and fluorescence pulse signals emitted by two classification fluoresceins used for microsphere classification and a calibration fluorescein;
• a fluorescence compensation module configured to perform fluorescence compensation on three pieces of fluorescence pulse data after the sampling so as to obtain true data of each fluorescence pulse, and map true data of two classification fluorescence pulses to a scatter plot so as to form a two-dimensional array;
• an ellipse gate constructing module configured to convert the two-dimensional array into a binary image, construct, based on a gathering region of each microsphere particle on the binary image, an ellipse gate related to the gathering region, and obtain intrinsic characteristics and movement characteristics of the ellipse gate, wherein the ellipse gate constructing module is specifically configured to perform the following steps:
• segmenting the formed binary image to obtain a connection domain in which each microsphere particle gathers;
• obtaining a relevant ellipse gate parameter based on an edge of the connection domain;
• and inversely converting the obtained ellipse gate parameter of each microsphere particle into a coordinate system corresponding to the two-dimensional array so as to obtain corresponding ellipse gate characteristics, wherein the ellipse gate characteristics comprise the intrinsic characteristic representing an ellipse gate size and the movement characteristic representing a transform from a standard position to the ellipse gate;
• an ellipse parameter obtaining module configured to obtain coordinates of two focuses on the ellipse gate based on the intrinsic characteristics and movement characteristics of each ellipse gate;
• a classification module configured to obtain the true data of two classification fluorescence pulses acquired on each microsphere particle so as to form coordinates of the microsphere particle, and compare a relationship between distances from coordinates of each microsphere particle to the two focuses on each ellipse gate and a major axis of the ellipse gate so as to classify the microsphere particle into a corresponding ellipse gate;
• and
• a calibration module configured to calculate a median value of true data of calibration fluorescence pulses on all of the microsphere particles within each ellipse gate for calibration or qualification.

A computer device, comprising a memory, a processor, and a computer program stored on the memory and capable of running on the processor, wherein when executing the computer program, the processor performs a method for analyzing flow matrix data, and the method comprises:

• emitting a beam on microsphere particles;
• acquiring and sampling a forward-scattered light pulse signal outputted by a flow matrix analyzer and fluorescence pulse signals emitted by two classification fluoresceins used for microsphere classification and a calibration fluorescein;
• performing fluorescence compensation on three pieces of fluorescence pulse data after the sampling so as to obtain true data of each fluorescence pulse, and mapping true data of two classification fluorescence pulses to a scatter plot so as to form a two-dimensional array;
• converting the two-dimensional array into a binary image, constructing, based on a gathering region of each microsphere particle on the binary image, an ellipse gate related to the gathering region, and obtaining intrinsic characteristics and movement characteristics of the ellipse gate, wherein this step comprises:
• segmenting the formed binary image to obtain a connection domain in which each microsphere particle gathers;
• obtaining a relevant ellipse gate parameter based on an edge of the connection domain;
• and inversely converting the obtained ellipse gate parameter of each microsphere particle into a coordinate system corresponding to the two-dimensional array so as to obtain corresponding ellipse gate characteristics, wherein the ellipse gate characteristics comprise the intrinsic characteristic representing an ellipse gate size and the movement characteristic representing a transform from a standard position to the ellipse gate;
• obtaining coordinates of two focuses on the ellipse gate based on the intrinsic characteristics and movement characteristics of each ellipse gate;
• obtaining the true data of two classification fluorescence pulses acquired on each microsphere particle so as to form coordinates of the microsphere particle, and comparing a relationship between distances from coordinates of each microsphere particle to the two focuses on each ellipse gate and a major axis of the ellipse gate so as to classify the microsphere particle into a corresponding ellipse gate;
• and
• calculating a median value of true data of calibration fluorescence pulses on all of the microsphere particles within each ellipse gate for calibration or qualification.