Method of DNA synthesis

An in vitro cell-free process for production of a deoxyribonucleic acid (DNA) molecule which comprises a desired DNA sequence, the process comprising:

• (a) contacting an immobilised oligonucleotide on a solid support with a series of template oligonucleotides which overlap in sequence, in the presence of at least one DNA polymerase under conditions promoting template-dependent extension of the immobilised oligonucleotide to produce a first DNA strand which comprises the desired DNA sequence and further comprises a first portion of a protelomerase target sequence proximal to the solid support, wherein the first DNA strand is a single-stranded molecule;
• (b) introducing a DNA sequence comprising a second portion of the protelomerase target sequence into a distal end of the first DNA strand, such that the first and second portions of the protelomerase target sequence thereby create a complete protelomerase target sequence proximal to the solid support;
• and
• (c) contacting the complete protelomerase target sequence proximal to the solid support with a protelomerase, to thereby produce and release the DNA molecule from immobilisation.