Multiple heteroresistance to guide combination antibiotic regimens
AssigneeEmory University
InventorsDavid Weiss, Victor Band, David Hufnagel
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Abstract
This disclosure contemplates detecting antimicrobial heteroresistance to guide antibiotic or other antimicrobial therapy. In certain embodiments, this disclosure contemplates avoiding a monotherapy with antibiotics to which an individual bacterial pie isolate is heteroresistant, because this can lead to treatment failure. Thus, in certain embodiments, one uses a combination of antibiotics to which a bacterium is individually heteroresistant.
Claims7
A method of determining whether a bacteria culture is heteroresistant to antibiotics and treating a bacterial infection with a combination of heteroresistant antibiotics comprising:
- obtaining a sample from a subject diagnosed with a bacterial infection;
- growing bacteria from said sample providing a purified single bacterial colony;
- contacting a plurality of individual antibiotics with samples of the purified single bacterial colony providing individual contacted bacterial cultures for each antibiotic in the library;
- determining whether the individual contacted bacterial cultures for each antibiotic in the library is either,
- i) resistant to the antibiotic,
- ii) heteroresistant to the antibiotic, or
- iii) susceptible to the antibiotic;
- and
- administering to said subject in need of treatment an effective amount of a combination of heteroresistant antibiotics for treatment, wherein each antibiotic in the combination of antibiotics for treatment is determined from the individual contacted bacterial cultures to be heteroresistant to each antibiotic in said combination of heteroresistant antibiotics for treatment.
Dependencies are inferred from the claim text.