Silk gland recombinant expression vector of silkworm (Bombyx mori) expressing human epidermal growth factor, preparation method and use thereof
AssigneeSouthwest University
InventorsDingpei Long, Xian Cheng, Jianfeng Sun, Yanping Liu, Fangyin Dai, Zhonghuai Xiang
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Abstract
Disclosed is a silk gland recombinant expression vector of silkworm expressing human epidermal growth factor, a preparation method and use thereof. The recombinant expression vector is formed by fusing truncated silk fibroin light chain N-terminal domain, C-terminal domain, optimized human epidermal growth factor and silkworm nuclear polyhedrosis virus enhancer, and removes the redundant part of the extra domain of endogenous silk protein contained in the fusion protein to the maximum extent.
Claims12
A silk gland recombinant expression vector of silkworm expressing human epidermal growth factor, wherein the silk gland recombinant expression vector of silkworm comprises a vector skeleton and a target gene expression frame, wherein the target gene expression frame comprises an optimized human epidermal growth factor gene sequence (EGF) and a silkworm nuclear polyhedrosis virus enhancer (hr3) sequence, and wherein the nucleotide sequences of the optimized human epidermal growth factor gene sequence and the silkworm nuclear polyhedrosis virus enhancer sequence are set forth in SEQ ID NO:3 and SEQ ID NO:1, respectively.
Dependencies are inferred from the claim text.