Methods for making L-glufosinate
AssigneeBASF SE
InventorsBrian Michael Green, Michelle Lorraine Gradley
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Abstract
Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.
Claims22
A method for making L-glufosinate, comprising:
- reacting D-glufosinate with a D-amino acid oxidase (DAAO) enzyme to form PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid) while aerating, wherein the DAAO enzyme has an activity of at least about 3 μmol/min*mg, wherein the reaction is conducted in the presence of a catalase;
- aminating the PPO to L-glufosinate by an L-amino acid dehydrogenase (LAAD) enzyme in the presence of an ammonia source and a redox cofactor in reduced form;
- and
- recycling the redox cofactor through reduction;
- wherein at least 70% of the D-glufosinate is converted to L-glufosinate;
- and
- wherein the method is performed ex vivo.
Dependencies are inferred from the claim text.