Materials and methods for protein production
AssigneeImpossible Foods Inc.
InventorsBiswajoy Roy Chaudhuri, Smita Shankar
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Abstract
This document relates to materials and methods for the production of protein. In one aspect, this document provides a first exogenous nucleic acid construct including a nucleotide sequence encoding an aminolevulinate synthase (ALAS) protein operably linked to a first promoter element, wherein the ALAS includes at least a first heme responsive motif (HRM), and wherein the ALAS includes a mutation in the first HRM, and a second exogenous nucleic acid construct comprising a nucleotide sequence encoding a heme-binding protein, wherein the second exogenous nucleic acid construct including a nucleotide sequence encoding the heme-binding protein is operably linked to the first promoter element or is operably linked to a second promoter element.
Claims12
A method of producing a heme-binding protein in a cell, the method comprising:
- expressing in the cell a first exogenous nucleic acid construct comprising a nucleotide sequence encoding an aminolevulinate synthase (ALAS) protein operably linked to a first promoter element, wherein the ALAS protein comprises an amino acid sequence having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:
- 29, wherein the ALAS protein comprises at least a first heme responsive motif (HRM), wherein the first HRM comprises a mutated C-P motif, and wherein the mutated C-P motif in the first HRM has a mutation from cysteine to a different amino acid;
- and
- expressing in the cell a second exogenous nucleic acid construct comprising a nucleotide sequence encoding a heme-binding protein,
- wherein the second exogenous nucleic acid construct comprising a nucleotide sequence encoding the heme-binding protein is operably linked to the first promoter element or is operably linked to a second promoter element.
Dependencies are inferred from the claim text.