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US 12,509,671 B2Granted
Mannanase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsVasudeva Prahlada Rao, Geetha Hiremath Mendez, Christian Isak Joergensen, Akshatha Venugopalan, Naveen Shivanand Raikar, Silja Poulose, Lars Olsen, Allan Svendsen, Jonatan Ulrik Fangel, Subith Krishna, Pengfei Tian, Rajendra Kulothungan Sainathan
Life of this patent
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Abstract
The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and detergent compositions and methods of using the variants.
Claims17
A variant of a parent mannanase, which comprises
- (a) a substitution with A, R, D, N, C, E, G, H, I, L, K, M, F, P, S, T or W or a deletion of Q, at the position corresponding to position 168 of SEQ ID NO:
- 1;
- and
- (b) a second substitution or a second deletion at one or more positions corresponding to positions selected from the group consisting of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19, 20, 21, 22, 23, 25, 26, 30, 35, 36, 37, 38, 42, 45, 46, 48, 49, 53, 57, 61, 63, 64, 65, 68, 69, 70, 72, 74, 76, 78, 82, 83, 86, 89, 94, 96, 97, 100, 101, 103, 105, 109, 110, 111, 112, 115, 117, 118, 120, 121, 122, 123, 126, 129, 130, 134, 137, 139, 141, 142, 143, 148, 155, 160, 161, 162, 163, 164, 165, 166, 167, 169, 170, 171, 172, 174, 176, 178, 179, 181, 182, 183, 184, 185, 188, 189, 190, 192, 193, 195, 197, 198, 210, 213, 214, 215, 217, 218, 219, 228, 235, 239, 242, 248, 249, 252, 253, 254, 258, 259, 260, 267, 270, 271, 272, 276, 280, 283, 286, 296, 299, 301, 304, 312, 315, 321, 322, 323, 324, 342, 344, 347, 351, 352, 354, 355, 358, 363, 366, 368, 377, 378, 379, 380, 385, 391, 398, 403, 408, 410, 413, 414, 417, 419, 422, 423, 437, 438, 440, 442, 443, 452, 454, 462, 463, 466, 467, 470, 472, 473, 477, 479, 480, 482, 483, 484, 486, 487, 488, 489, 490, and 491 of SEQ ID NO:
- 1;
- wherein the variant has mannanase activity and has at least 85% sequence identity to the amino acid sequence of SEQ ID NO:
- 1.
4 more elements
Dependencies are inferred from the claim text.