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US 12,509,491 B2Granted
Mnep monomer variant and application thereof
AssigneeQitan Technology LTD., Beijing
InventorsShaowei Liu, Ya Zhou, Chengyao Chen
Life of this patent
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Abstract
The present invention provides a Mnep monomer variant including an amino acid sequence with any one or more amino acid mutations at positions 92-104 of SEQ ID NO: 1, a porin or construct including at least one Mnep monomer variant, and a use thereof. The present invention also provides a method for characterizing a target polynucleotide.
Claims18
A Mnep monomer variant, comprising a sequence that is at least 94% identical to SEQ ID NO:
- 1, wherein either one or more of the following are true:
- (a) an amino acid at a position corresponding to position 92 of SEQ ID NO:
- 1 is not glycine (G);
- (b) an amino acid at a position corresponding to position 93 of SEQ ID NO:
- 1 is not aspartic acid (D);
- (c) an amino acid at a position corresponding to position 94 of SEQ ID NO:
- 1 is not proline (P);
- (d) an amino acid at a position corresponding to position 95 of SEQ ID NO:
- 1 is not glycine (G);
- (e) an amino acid at a position corresponding to position 96 of SEQ ID NO:
- 1 is not phenylalanine (F);
- (f) an amino acid at a position corresponding to position 97 of SEQ ID NO:
- 1 is not glycine (G);
- (g) an amino acid at a position corresponding to position 98 of SEQ ID NO:
- 1 is not isoleucine (I);
- (h) an amino acid at a position corresponding to position 99 of SEQ ID NO:
- 1 is not threonine (T);
- (i) an amino acid at a position corresponding to position 100 of SEQ ID NO:
- 1 is not glycine (G);
- (j) an amino acid at a position corresponding to position 101 of SEQ ID NO:
- 1 is not isoleucine (I);
- (k) an amino acid at a position corresponding to position 102 of SEQ ID NO:
- 1 is not glycine (G);
- (l) an amino acid at a position corresponding to position 103 of SEQ ID NO:
- 1 is not glycine (G);
- and
- (m) an amino acid at a position corresponding to position 104 of SEQ ID NO:
- 1 is not alanine (A).
25 more elements
A porin comprising at least one Mnep monomer variant, wherein the at least one Mnep monomer variant comprises a sequence that is at least 94% identical to SEQ ID NO:
- 1, wherein the sequence comprises one or more of the following mutations:
- (a) an amino acid at a position corresponding to position 92 of SEQ ID NO:
- 1 is not glycine (G);
- (b) an amino acid at a position corresponding to position 93 of SEQ ID NO:
- 1 is not aspartic acid (D);
- (c) an amino acid at a position corresponding to position 94 of SEQ ID NO:
- 1 is not proline (P);
- (d) an amino acid at a position corresponding to position 95 of SEQ ID NO:
- 1 is not glycine (G);
- (e) an amino acid at a position corresponding to position 96 of SEQ ID NO:
- 1 is not phenylalanine (F);
- (f) an amino acid at a position corresponding to position 97 of SEQ ID NO:
- 1 is not glycine (G);
- (g) an amino acid at a position corresponding to position 98 of SEQ ID NO:
- 1 is not isoleucine (I);
- (h) an amino acid at a position corresponding to position 99 of SEQ ID NO:
- 1 is not threonine (T);
- (i) an amino acid at a position corresponding to position 100 of SEQ ID NO:
- 1 is not glycine (G);
- (j) an amino acid at a position corresponding to position 101 of SEQ ID NO:
- 1 is not isoleucine (I);
- (k) an amino acid at a position corresponding to position 102 of SEQ ID NO:
- 1 is not glycine (G);
- (l) an amino acid at a position corresponding to position 103 of SEQ ID NO:
- 1 is not glycine (G);
- and
- (m) an amino acid at a position corresponding to position 104 of SEQ ID NO:
- 1 is not alanine (A),
- and wherein the one or more mutations result in differences in resistance in a pore due to differences in physical or chemical properties of different types of nucleotides when a single polynucleotide strand passes through the porin comprising at least one Mnep monomer variant.
26 more elements
Dependencies are inferred from the claim text.