# US 12,508,608 B2 · Method of depositing a substance on a substrate > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,508,608 B2 - Document ID: US12508608B2 - Status: Granted - Kind: B2 - Application number: 17/622,215 - Filed: 2020-06-26 - Published: 2022-12-29 - Granted: 2025-12-30 - Earliest claimed: 2019-07-01 - Assignees: DBV Technologies; UCL Business PLC - Applicants: Dbv Technologies; Ucl Business Ltd - Inventors: Nael Berri; Sarah Darwiche; Maxime Huet; Suwan Jayasinghe; Adam Wojcik - Web page: https://patentgrep.com/patent/US12508608B2 ## CPC - A61K 8/0208: {Tissues; Wipes; Patches} - B05B 5/0255: {spraying and depositing by electrostatic forces only} - A61K 8/64: Proteins; Peptides; Derivatives or degradation products thereof - A61K 9/70: Web, sheet or filament bases {; Films; Fibres of the matrix type containing drug (hollow drug-filled fibres A61K9/0092)} - A61K 9/7023: {Transdermal patches and similar drug-containing composite devices, e.g. cataplasms (galenical aspects of iontophoretic devices A61K9/0009; microneedle arrays A61K9/0021; buccal patches A61K9/006)} - A61Q 19/00: Preparations for care of the skin - B05B 5/03: characterised by the use of gas {, e.g. electrostatically assisted pneumatic spraying (B05B5/04, B05B5/043, B05B5/047 take precedence)} - A61K 2800/10: General cosmetic use - A61K 2800/805: Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95 - A61M 2037/0053: {Methods for producing microneedles} - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - B05B: SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES (spray-mixers with nozzles B01F25/72; processes for applying liquids or other fluent materials to surfaces by spraying B05D) - A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS - A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)} ## Abstract The invention relates to a method of depositing a substance onto a substrate (6), comprising: —providing a substrate (6) at a distance from a conducting spraying nozzle (1), said spaying nozzle (1) having an outlet (2); —providing a liquid composition containing the substance to the spraying nozzle (1); —generating electrically charged liquid droplets from the liquid composition between the outlet (2) of the spraying nozzle (1) and the substrate (6), by providing compressed gas around the liquid composition flowing out of the outlet (2) of the spraying nozzle (1) and by providing an electric field downstream of the outlet (2) of the spraying nozzle (1); and—collecting the generated liquid droplets on the substrate (6). The invention also relates to an installation for carrying out this method. ## Claims ### Claim 1 A method of depositing one or more allergens onto a substrate, comprising: providing a substrate at a distance from a conducting spraying nozzle, said spraying nozzle having an outlet; providing a liquid composition containing the one or more allergens to the spraying nozzle at a flow rate of from 3 to 50 mL/h; generating electrically charged liquid droplets from the liquid composition between the outlet of the spraying nozzle and the substrate, by providing compressed gas around the liquid composition flowing out of the outlet of the spraying nozzle and by providing an electric field downstream of the outlet of the spraying nozzle, wherein the compressed gas is selected from the group consisting of nitrogen, nitrous oxide, carbon dioxide, inert gas, argon, and mixtures thereof, and further wherein the compressed gas around the liquid composition is at a pressure of from 0.1 to 2 bar above atmospheric pressure; and collecting the generated liquid droplets on the substrate; wherein the spraying nozzle is arranged within a chamber, the compressed gas is fed to the chamber, and the liquid composition and compressed gas flow out of the chamber through an ejection opening in a conducting ejector, and wherein the one or more allergens are selected from the group consisting of peanut, milk, egg, walnut, cashew nut, pecan nut, pistachios, and hazelnut allergens. ### Claim 2 (depends on 1) The method of claim 1, wherein the liquid composition has a conductivity of from 0.1 μS/m to 3 S/m. ### Claim 3 (depends on 1) The method of claim 1, wherein an electric field having a first magnitude is applied in a first area between the outlet of the spraying nozzle and the ejection opening; and/or an electric field having a second magnitude is applied in a second area between the ejection opening and the substrate; and wherein the first magnitude is from 0.1 to 10 MV/m; and/or the second magnitude is from 0.02 to 1 MV/m; and/or the first magnitude is larger than the second magnitude. ### Claim 4 (depends on 1) The method of claim 1, which does not comprise any drying step after collecting the droplets on the substrate. ### Claim 5 (depends on 1) The method of claim 1, for making one selected from the group consisting of a patch and a therapeutic patch. ### Claim 6 (depends on 1) The method of claim 1, wherein the compressed gas around the liquid composition is at a pressure of from 0.3 to 1 bar above atmospheric pressure. ### Claim 7 (depends on 3) The method of claim 3, wherein the first magnitude is from 0.5 to 5 MV/m; and/or the second magnitude is from 0.1 to 0.5 MV/m. ### Claim 8 (depends on 1) The method of claim 1, wherein the liquid composition is provided to the spraying nozzle at a flow rate selected from the group consisting of 3 to 5 mL/h, 3 to 10 mL/h, 3 to 20 mL/h, 5 to 10 mL/h, 5 to 20 mL/h, 5 to 50 mL/h, 10 to 20 mL/h, 10 to 50 mL/h, and 20 to 50 mL/h. ### Claim 9 (depends on 1) The method of claim 1, wherein the compressed gas is selected from the group consisting of nitrogen, nitrous oxide, and mixtures thereof. ### Claim 10 (depends on 1) The method of claim 1, wherein the one or more allergens are selected from the group consisting of peanut, milk, and egg allergens. ### Claim 11 (depends on 1) The method of claim 1, wherein the one or more allergens is peanut allergen. ### Claim 12 (depends on 1) The method of claim 1, wherein the one or more allergens is milk allergen. ### Claim 13 (depends on 1) The method of claim 1, wherein the one or more allergens is egg allergen.