Integrated synthesis and analysis systems and methods of use thereof

A method for forming a chemical product and conducting one or more biological assays on the chemical product, the method comprising:

• forming a chemical product in a desorption electrospray ionization (DESI) droplet product from directing a DESI spray from a DESI source onto a substrate to desorb and ionize chemical molecules and form the chemical product, wherein the forming step is conducted for each spot from a surface of an array of spots;
• analyzing the chemical product in a mass spectrometer;
• collecting the chemical product;
• incubating the chemical product with one or more biological molecules;
• and
• analyzing results of the incubation of the chemical product with the one or more biological molecules;
• wherein all steps of the method are performed by an integrated synthesis and analysis system that comprises a fluid handling apparatus comprising one or more robotic instruments configured to handle and move the chemical product through each step of the method and a control module comprising software to control and coordinate operation of each of the method steps.