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US 12,504,353 B2Granted
Cell manipulation device and cell manipulation method
AssigneeNikon Corporation
InventorsMasaki Moriyama, Takeshi Kawano
Life of this patent
Estimating the term…
Abstract
A cell manipulation method is provided including: culturing cells in a liquid; disposing a flow path through which a gas is able to be introduced in the liquid; forming an air bubble at an end portion of the flow path; and attaching the cells to the air bubble.
Claims6
A cell manipulation device, comprising:
- a flow path, an end portion of which is configured to be disposed in a liquid and through which a gas is introduced into the liquid, the liquid being a liquid within which cells on a solid phase are cultured, and
- a control unit for controlling the operation of the flow path, the control unit being configured to
- (i) form an air bubble that extends out of the end portion of the flow path,
- (ii) control a volume of the air bubble that extend out of the end portion of the flow path for allowing the air bubble to be maintained and to remain in contact with the solid phase while the flow path having the air bubble extending therefrom is moved across a surface the solid phase for allowing cells on the surface of the solid phase to attach to the air bubble, and
- (iii) collect the cells that adhere to the air bubble through the flow path.
2 more elements
Dependencies are inferred from the claim text.