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US 12,503,711 B2Granted
Method of producing and processing diamines to a diamine free base using a carbonate intermediate and an engineered microorganism
AssigneeGenomatica, Inc.
InventorsLauri H. Suominen, Connor J. Galleher, Michael Japs, Mark J. Burk, Cara Tracewell
Life of this patent
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Abstract
Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.
Claims18
A method for diamine (DA) production comprising steps of:
- a) culturing a genetically engineered microorganism in a medium under suitable conditions and for a sufficient period of time form a cultured medium comprising one or more of DA carbonate, DA bicarbonate, or DA bis-bicarbonate, and/or DA carbamate or DA biscarbamate, wherein carbon dioxide, carbonate, bicarbonate or carbonic acid predominantly control pH of the medium, the microorganism comprising at least one exogenous nucleic acid encoding at least one enzyme of a DA synthesis pathway, and wherein the diamine comprises C2 to C7 methylene segments or C2 to C12 methylene segments;
- b) converting at least one or more of the DA carbonate, DA bicarbonate, or DA bis-bicarbonate, and/or DA carbamate or DA biscarbamate, into DA free base and carbon dioxide;
- and;
- c) isolating the DA free base;
- wherein the DA synthesis pathway is a hexamethylenediamine synthesis pathway, a cadaverine synthesis pathway, a putrescine synthesis pathway, an ethylenediamine synthesis pathway, or a heptamethylenediamine synthesis pathway;
- and
- wherein the at least one exogenous nucleic acid encoding at least one enzyme of a DA synthesis pathway comprises an exogenous nucleic acid encoding 3-oxoadipyl-CoA thiolase, 6-ACA transaminase or dehydrogenase, 6-aminocaproyl-CoA reductase, 6-ACA reductase, adipyl-CoA reductase, adipate reductase, 6-hydroxy 3-oxohexanoyl-CoA dehydrogenase, 2-aminopimelate decarboxylase, or homolysine decarboxylase, or combinations thereof.
4 more elements
A method for diamine (DA) production comprising the steps of:
- a) culturing a genetically engineered microorganism in a medium under suitable conditions and for a sufficient period of time to form a cultured medium comprising one or more of DA carbonate, DA bicarbonate, DA bis-bicarbonate, DA carbamate or DA biscarbamate, wherein the diamine comprises C2 to C7 methylene segments or C2 to C12 methylene segments, and wherein the microorganism comprises at least one exogenous nucleic acid encoding at least one enzyme of a DA synthesis pathway, wherein percent dissolved inorganic carbon (DIC) in the medium is greater than or equal to 40%;
- and the DIC is determined by the formula:
- DIC/TDCA×100
- wherein TDCA is the Total Dissolved Counter Anions and is the sum of DIC and other anions,
- b) converting the DA carbonate, DA bicarbonate, DA bis-bicarbonate, DA carbamate or DA biscarbamate into DA free base and carbon dioxide;
- and
- c) isolating the DA free base.
4 more elements
A method for diamine (DA) production comprising the steps of:
- a) culturing a genetically engineered microorganism in a medium under suitable conditions and for a sufficient period of time to form a cultured medium comprising one or more of DA carbonate, DA bicarbonate, DA bis-bicarbonate (DA Carbonates) and/or DA carbamate or DA biscarbamate (DA Carbamates) in the medium, wherein at least 40% of diamine species in the medium comprises one or more of DA carbonate, DA bicarbonate, DA bis-bicarbonate, DA carbamate or DA biscarbamate, and wherein the microorganism comprises at least one exogenous nucleic acid encoding at least one enzyme of a DA synthesis pathway, and wherein the diamine comprises C2 to C7 methylene segments or C2 to C12 methylene segments;
- b) converting the DA Carbonates and/or DA Carbamates into DA free base and carbon dioxide;
- and
- c) isolating the DA free base.
1 more element
Dependencies are inferred from the claim text.