Base editing enzymes
AssigneeMetagenomi, Inc.
InventorsBrian C. Thomas, Jyun-Liang Lin, Alan Brooks, Cristina Butterfield, Christopher Brown, Cindy Castelle, Morayma Temoche-Diaz, Benjamin Freeman, Christine Romano, Rebecca Lamothe
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Abstract
The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof.
Claims30
An engineered nucleic acid editing system comprising:
- (a) a cytidine deaminase or a nucleic acid encoding said cytidine deaminase, wherein said cytidine deaminase comprises a sequence having at least 80% sequence identity to any one of SEQ ID NOs:
- 774 or 835;
- and
- (b) an endonuclease domain or a nucleic acid encoding said endonuclease domain, wherein said cytidine deaminase is fused or linked to said endonuclease domain.
A method of deaminating a cytosine residue in a nucleic acid sequence in a eukaryotic cell, said method comprising contacting to said nucleic acid sequence a polypeptide comprising a cytidine deaminase, wherein said cytidine deaminase comprises a sequence having at least 80% sequence identity to any one of SEQ ID NOs:
- 774 or 835.
A method of editing a TRAC locus in a eukaryotic cell, said method comprising contacting said TRAC locus with a polypeptide comprising a cytidine deaminase, wherein said cytidine deaminase comprises a sequence having at least 80% sequence identity to any one any one of SEQ ID NOs:
- 774 or 835, thereby editing said TRAC locus in said eukaryotic cell.
Dependencies are inferred from the claim text.