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US 12,503,694 B2Granted
Systems and methods for biomolecule retention
AssigneeNautilus Subsidiary, Inc.
InventorsTorri Elise Rinker, Christina Inman, Steven Tan, Pengyu Hao, Elvis Ikwa, Pierre Indermuhle, Parag Mallick, Tural Aksel, Stephen Hendricks, Sadie Ingle
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Abstract
Compositions, systems, and methods for the display of analytes such as biomolecules are described. Display of analytes is achieved by coupling of the analytes to displaying molecules that are configured to associate with surfaces or interfaces. Arrays of analytes may be formed from the described systems for utilization in assays and other methods.
Claims35
A method, comprising:
- a) providing a solid support comprising a plurality of sites, wherein each individual site of the plurality of sites comprises:
- i) only one particle coupled to the individual site;
- and
- ii) only one polypeptide of interest attached to the only one particle;
- b) delivering binding reagents to the solid support, thereby coupling binding reagents to polypeptides of interest at sites of the plurality of sites;
- c) repeating step b) using second binding reagents instead of the binding reagents, wherein the second binding reagents are different from the binding reagents;
- d) for each individual site, detecting presence or absence of coupling of each individual binding reagent and second binding reagent to the polypeptide of interest;
- and
- e) based upon presence or absence of coupling of each individual binding reagent and second binding reagent to the polypeptide of interest, characterizing the polypeptide of interest at each individual site of the plurality of sites.
6 more elements
Dependencies are inferred from the claim text.