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US 12,503,675 B2Granted
Genetically engineered yeast cells and methods of use thereof
AssigneeBerkeley Fermentation Science Inc.
InventorsJeremy Roop, Charles Denby, Rachel Li, Nicholas Harris
Life of this patent
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Abstract
Provided herein are genetically modified yeast cells that recombinantly expresses a gene encoding a mutant beta-lyase. Also provided are methods of producing fermented products and methods of producing ethanol.
Claims20
A genetically modified yeast cell, comprising a heterologous gene encoding a beta-lyase variant having beta-lyase activity that, when used for fermenting unhopped wort:
- (i) produces less than 150 μg/L indole and at least 200 ng/L 3-mercaptohexan-1-ol (3MH);
- or
- (ii) produces at least a 5-fold increased concentration of 3MH fold, as compared to a cell from which the genetically modified yeast cell is derived, and produces an indole concentration that is reduced by at least 5% as compared to a genetically modified yeast cell comprising wild-type beta-lyase.
Dependencies are inferred from the claim text.