US 12,502,665 B2 · On-flow cell three dimensional polymer structures

Abstract

A method for making on-flow cell three-dimensional polymer structures includes loading a polymer precursor solution onto a flow cell. The polymer precursor solution includes a monomer, a crosslinker, and a photoinitiator. The flow cell includes at least one channel for receiving the polymer precursor solution. The at least one channel has an upper interior surface and a lower interior surface. The method further includes illuminating the polymer precursor solution through a patterned photomask using a light at a wavelength sufficient to activate the photoinitiator. Activation of the photoinitiator polymerizes at least some of the polymer precursor solution underneath apertures in the patterned photomask and forms three-dimensional polymer structures that extend from the upper interior surface to the lower interior surface of the at least one channel.

Claims20

Dependent claims

A method for making on-flow cell three-dimensional polymer structures with biological cells or genetic material embedded in the three-dimensional polymer structures, comprising:

loading a polymer precursor solution onto a flow cell,
wherein the polymer precursor solution includes the biological cells or genetic material,
a monomer, a crosslinker, and a photoinitiator,
wherein the flow cell includes at least one channel for receiving the polymer precursor solution, and
wherein the at least one channel has an upper interior surface defined by an upper flow cell substrate and a lower interior surface defined by a lower flow cell substrate;
and
illuminating the polymer precursor solution through the upper flow cell substrate via a patterned photomask using a light at a wavelength sufficient to activate the photoinitiator, wherein activation of the photoinitiator polymerizes at least some of the polymer precursor solution underneath apertures in the patterned photomask and forms three-dimensional polymer structures in which the biological cells or genetic material is embedded that extend from the upper interior surface to the lower interior surface of the at least one channel.

4 more elements

A method for sequencing in three-dimensions using an on flow-cell three-dimensional sequencing matrix, comprising:

loading a polymer precursor solution into a flow cell, wherein the polymer precursor solution includes monomers and oligonucleotides;
polymerizing the polymer precursor solution to create a permeable three-dimensional polymer matrix within the flow cell;
diffusing a sequencing library into the permeable three-dimensional polymer matrix,
wherein the sequencing library includes nucleic acid fragments;
diffusing enzymes and reagents into the permeable three-dimensional polymer matrix;
hybridizing the nucleic acid fragments to the oligonucleotides in the permeable three-dimensional polymer matrix to produce hybridized nucleic acid fragments;
clonally amplifying the hybridized nucleic acid fragments to create clusters for sequencing within the permeable three-dimensional polymer matrix;
sequencing the clusters within the permeable three-dimensional polymer matrix;
and
optically imaging the sequenced clusters within the three-dimensional polymer matrix in multiple, discrete two-dimensional slices to characterize the sequencing library, wherein the multiple, discrete two-dimensional slices represent the entire three-dimensional internal volume of the flow cell.

7 more elements

Dependencies are inferred from the claim text.

On-flow cell three dimensional polymer structures

Life of this patent

On-flow cell three dimensional polymer structures

Life of this patent

Abstract

Claims20