Antibacterial phage, therapeutic composition, bactericidal agent, food, bacteria identification kit, therapeutic composition manufacturing method, bacteria elimination method, bacteria identification method, and animal therapeutic method

An antibacterial phage including

• CRISPR-Cas13a having a targeting sequence that is complementary to a specific gene as a target and suppressing growth and causing bactericidal action to bacteria, wherein
• the specific gene is a drug resistance gene, a pathogenic gene, or a toxin gene;
• and
• the target sequence includes:
• SEQ ID NO:
• 7, SEQ ID NO:
• 17, SEQ ID NO:
• 22, SEQ ID NO:
• 26, SEQ ID NO:
• 27, SEQ ID NO:
• 28, SEQ ID NO:
• 36, SEQ ID NO:
• 37, SEQ ID NO:
• 38, SEQ ID NO:
• 39, SEQ ID NO:
• 40, SEQ ID NO:
• 41, SEQ ID NO:
• 42, SEQ ID NO:
• 43, SEQ ID NO:
• 44, SEQ ID NO:
• 45, SEQ ID NO:
• 46, SEQ ID NO:
• 47, SEQ ID NO:
• 48, SEQ ID NO:
• 49, SEQ ID NO:
• 50, SEQ ID NO:
• 51, or SEQ ID NO:
• 52.

A therapeutic composition manufacturing method comprising the steps of:

• transforming and/or infecting a phagemid having a packaging sequence, Cas13a, and CRISPR with a target sequence that recognizes a specific gene as a target, and helper phage with a phage-synthesizing bacteria, and lysing and/or releasing out of the cell;
• and
• obtaining constructed antibacterial phage suppressing growth and causing bactericidal action to bacteria;
• wherein the specific gene is selected from IMP-1, KPC-2, NDM-1, OXA-48, VIM-2, mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5.

A bacteria elimination method comprising the steps of:

• applying antibacterial phage including CRISPR-Cas13a having a target sequence that recognizes a specific gene as a target;
• and
• eliminating bacteria having the specific gene by causing bactericidal action to the bacteria;
• wherein the specific gene is selected from IMP-1, KPC-2, NDM-1, OXA-48, VIM-2, mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5.

A bacteria identification method comprising the steps of:

• infecting bacteria with antibacterial phage including CRISPR-Cas13a having a target sequence that recognizes a specific gene as a target;
• and
• determining and/or testing whether the bacteria have the specific gene by suppressing growth and causing bactericidal action to the bacteria, wherein
• the specific gene is a drug resistance gene, a pathogenic gene, or a gene for discriminating the bacteria;
• and
• the specific gene is selected from IMP-1, KPC-2, NDM-1, OXA-48, VIM-2, mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5.