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US 12,501,868 B2Granted
Haploid induction compounds and methods for use thereof
AssigneeWimi Biotechnology (Hainan) Co., Ltd.
InventorsJianbing Yan, Xiang Li, Chenglin Jiang
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Abstract
A method for selecting a compound of haploid induction is provided. The method includes: treating a reproductive tissue of a plant with a reagent containing compounds to be tested; detecting a reactive oxygen species (ROS) level in the reproductive tissue after the treatment; and determining a compound that leads to an increase of the ROS level as the compound.
Claims11
A method for inducing haploid seed production using a reagent that comprises a compound, wherein the compound is lecithin from soybean and with CAS No. 8002-43-5, comprising:
- (a) dissolving or emulsifying the compound into the reagent;
- and
- (b) treating a reproductive tissue of a plant with the haploid-inducing reagent, wherein the treatment occurs immediately preceding, during or immediately following pollination, and at least one haploid seed is produced,
- wherein the haploid induction ability of the compound results from an increase of a reactive oxygen species (ROS) level in a reproductive tissue of a plant caused by the compound, thereby increasing the haploid-induction rate compared to control seeds that are not treated with the reagent.
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Dependencies are inferred from the claim text.