Apparatus for controlling positive and negative pressure or flow in a fluidic system

A method of controlling pressure or flow in a fluidic system, wherein said fluidic system is a microfluidic or nanofluidic system, wherein said fluidic system is fluidically connected to the main inlet/outlet of an apparatus comprising

• a main inlet/outlet being configured to be fluidically connected to the fluidic system or to a reservoir of fluid connected to said fluidic system;
• a uni-directional pumping device configured to pump gas from an inlet of the uni-directional pumping device to an outlet of the uni-directional pumping device;
• a valves array;
• and
• a control unit configured to control the valves array and the pumping device;
• wherein the control unit is configured to set the valves array into at least two states comprising:
• a first state in which a first gas source is fluidically connected to the inlet of the uni-directional pumping device, and the outlet of the uni-directional pumping device is fluidically connected to the main inlet/outlet;
• and
• a second state in which the main inlet/outlet is fluidically connected to the inlet of the pumping device, and the outlet of the pumping device is fluidically connected to a second gas source,
• the method comprising adjusting one or more of said uni-directional pumping device, and said valves array.